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BioTek Microplate Washers  Product Detail
Model: ELx405

The ELx405's new patent pending Ultrasonic Advantage™ offers effortless washer manifold maintenance. Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. Cleaning these tubes is a time consuming and labor intensive process. BioTek's Ultrasonic Advantage™ incorporates the power of ultrasonic cleaning to make the ELx405 the only self-maintaining microplate washer available today.

The ELx405 HT is designed specifically to rapidly wash 384-well microplates and includes BioTek's patented Dual-Action™ manifold to ensure optimal performance and easy maintenance.

The ELx405 Select is the market leader in 96-/384-well washing. The Select also has our patented Dual-Action™ manifold, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection in both 96- and 384-well microplates. This unique design washes both 96- and 384-well plates without the need for any hardware changes. With its slanted dispense tubes, full control of dispense and aspiration rates and unique x, y, z positioning, the ELx405 Select is ideal for the most demanding wash requirements. The ELx405 Select CW is specially designed to accommodate non- or loosely-adherent cell lines.

For those applications that use only 96-well microplates, the standard ELx405 Microplate Washer is an ideal choice for performance and value! The ELx405 is the most precise and reliable washer available. Many unique features lower background absorbance values and increase the signal-to-noise ratio of typical ELISA assays.

The ELx405 Magna is specifically designed for those applications using magnetic bead technology. A second carrier positions the magnet plate close to the microplate bottom, securing magnetic beads during the critical aspiration cycles. All ELx405 Series washers come standard with BioTek's unique AutoPrime™ and AutoRinse™ functions for reliable performance, and can be interfaced into standard robotic systems with BioTek's easy-to-use ActiveX™ component.

Features:
  • Hardware

    • Patented Dual-Action™ manifold on the ELx405™ Select and ELx405™ HT provides independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection

    • Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir

    • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for 'blank' plates when used in automated robotic systems

    • Fully variable dispense volumes, flow, and aspiration rates provide 'total control' of the washing cycles from a gentle cell-washing application to vigorous wash for high-binding assays

    • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation

    • Optional external valve module connects four reagent bottles with automatic switching between reagents

  • Software:

    • 75 programs

    • Link up to 10 programs (if valve module is attached, links multiple buffers automatically)

    • Pre/AutoPrime

    • 10 cycles/wash

    • Crosswise aspiration well

    • Fully programmable:

      • Dispense height

      • Aspiration height

      • Aspiration delay

      • Soak

      • Shake

      • Aspiration rate

      • Horizontal aspirate and dispense position

    • External programming/downloading available through RS-232 port

Specifications:
  • Microplate types: All standard flat-, round-, or V-bottom plates/strips in 96-well array, 384- or 96-well plates (Select), 96-well plate (ELx405/Magna), 384-well plate (HT)

  • Processing speed: <20 seconds (1 cycle, 400 µl/well, 384 wells), <30 seconds (3 cycles, 300 µl/well, 96 wells), <80 seconds (3 cycles, 100 µl/well, 384 wells)

  • Wash cycles: 1-10

  • Volume range: 50 to 3000 µl/well, 25 to 3000 µl/well (HT)

  • Fluid delivery: Internal positive displacement pump

  • Buffer selection: Valve module option - up to 4 buffers

  • Dispense precision: <3.0% CV (96 wells)

  • Residual volume: <2.0 µl/well (average, 96 wells)

  • Sterilization: Chemical

  • Soak Time: 1 to 600 seconds

  • Shaking: User-programmable speeds and timing

  • Manifold: 96-channel aspiration/dispense (ELx405/Magna), 96-channel patented Dual-Action, with separate dispense/aspiration (Select), 192-channel patented Dual-Action, with separate dispense/aspiration (HT)

  • Supply bottle volume: 3.7 liter

  • Onboard software:

    • 75 programs

    • Program linking

    • Overflow wash/overfill protection

    • 5 maintenance programs

  • PC software: ActiveX component (optional)

  • Power: 100 to 240 VAC, 50/60 Hz

  • Dimensions: 17" D x 14" W x 9" H (43.2 x 35.6 x 22.9 cm)

  • Weight: 28 lbs (12.5 kg)

  • Regulatory: IVD (In Vitro Diagnostics) Directive. All BioTek microplate instrumentation is CE and NRTL Safety Agency marked.

  • Automation: Compatible with Bio-Stack Microplate Stacker and Bio-Stack Twister II Microplate Handler.



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